Recombinant Human DNA-damage-inducible Transcript 3

• Cat.No.: DDIT3-130H
• Product Overview: Recombinant Human DNA-damage-inducible transcript 3 protein expressed inE.coliencodes full length amino acids and has a molecular mass of 52 kDa.

Specification

• Cat. No.: DDIT3-130H
• Description: GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. GADD153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and merabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites.
• Source: E.coli.
• Formulation: Recombinant Human GADD153 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application: •ELISA •Inhibition Assays •Western Blotting.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 52 kDa.
• Storage & Stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
• Publication:
  Is there a common upstream link for autophagic and apoptotic cell death in human high-grade gliomas? (2011)

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Synonyms: DDIT3; DNA-damage-inducible transcript 3; CHOP; CEBPZ; CHOP10; GADD153; MGC4154; C/EBP zeta; DDIT-3; Growth arrest and DNA-damage-inducible protein GADD153; C/EBP-homologous protein; C/EBP homologous protein; CCAAT/enhancer-binding protein homologous protein; growth arrest- and DNA damage-inducible
• Gene ID: 1649
• mRNA Refseq: NM_004083
• Protein Refseq: NP_004074
• MIM: 126337
• UniProt ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: MAPK signaling pathway; Diabetes pathways
• Function: protein dimerization activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity