Active Recombinant Human NCR3LG1 protein(Met1-Ser262), hFc-tagged

• Cat.No.: NCR3LG1-546H
• Product Overview: Recombinant Human NCR3LG1 (NP_001189368.1) (Met1-Ser262) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Ser262
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human NCR3 at 10μg/mL (100μL/well) can bind human B7-H6, the EC50 of human B7-H6 is 20-100ng/mL.
• Molecular Mass: The recombinant human NCR3LG1 consists 476 amino acids and predicts a molecular mass of 53.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.