Recombinant Human HHLA2 protein, His-tagged HHLA2-549H

Recombinant Human HHLA2 protein, His-tagged

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Recombinant Human HHLA2 protein, His-tagged

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Cat.No. : HHLA2-549H
Product Overview : Recombinant Human HHLA2 (Ile23-Asn344) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Ile23
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 38.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Ile23-Asn344
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of HHLA2-549H :

Human B7-H7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of HHLA2-549H :

Immobilized Human CD28H, Fc Tag at 5 μg/mL (100 μL/well) can bind Human B7-H7, His Tag with a linear range of 0.02-0.313 μg/mL (QC tested).

Bioactivity-ELISA :

Immobilized Human B7-H7, His Tag at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag with a linear range of 1-20 ng/mL (Routinely tested).

Gene Name : HHLA2
Official Symbol : HHLA2
Synonyms : HHLA2; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; human endogenous retrovirus-H long terminal repeat-associating protein 2
Gene ID : 11148
mRNA Refseq : NM_007072
Protein Refseq : NP_009003
MIM : 604371
UniProt ID : Q9UM44

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