||Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques, 21.1kDa (189aa).
||Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.coli is a periplasmic protein and thioredoxin superfamily member whichintroduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids).
|Sequences of amino acids:
||AQYEDGKQYT TLEKPVAGAP QVLEFFSFFC PHCYQFEEVL HISDNVKKKL PEGVKMTKYH VNFMGGDLGK DLTQAWAVAM ALGVEDKVTV PLFEGVQKTQ TIRSASDIRD VFINAGIKGE EYDAAWNSFV VKSLVAQQEK AAADVQLRGV PAMFVNGKYQ LNPQGMDTSN MDVFVQQYAD TVKYLSEKK
||Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 2 mM EDTA
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.