Recombinant E.coli Disulfide Oxidoreductase A

• Cat.No.: DsbA-80E
• Product Overview: Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques, 21.1kDa (189aa).

Specification

• Cat. No.: DsbA-80E
• Description: Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.coli is a periplasmic protein and thioredoxin superfamily member whichintroduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids).
• Sequences of amino acids: AQYEDGKQYT TLEKPVAGAP QVLEFFSFFC PHCYQFEEVL HISDNVKKKL PEGVKMTKYH VNFMGGDLGK DLTQAWAVAM ALGVEDKVTV PLFEGVQKTQ TIRSASDIRD VFINAGIKGE EYDAAWNSFV VKSLVAQQEK AAADVQLRGV PAMFVNGKYQ LNPQGMDTSN MDVFVQQYAD TVKYLSEKK
• Formulation: Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 2 mM EDTA
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: dsbA periplasmic protein disulfide isomerase I [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: dsbA; Disulfide Oxidoreductase A; dsf; ECK3852; iarA; JW3832; ppfA; periplasmic protein disulfide isomerase I; Thiol:disulfide interchange protein dsbA
• Gene ID: 948353
• Protein Refseq: NP_418297
• UniProt ID: P0AEG4