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Recombinant Rat Thioredoxin Reductase 1

Cat.No.: Txnrd1-126R
Description: This gene encodes a member of the family of pyridine nucleotide-disulfide oxidoreductases. This protein is a flavoenzyme, which uses NADPH for reduction of thioredoxins as well as other protein and nonprotein substrates, and plays a role in protection against oxidative stress. It contains a selenocysteine (Sec) residue, which is essential for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal.
Source: E. coli
Species: Rat
Bio-activity: One ampoule contains 340 µl of recombinant rat thioredoxin reductase 1 (TrxR1) in 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 and 50 percent glycerol with a total activity of 10.5 units and 0.3 mg of active enzyme protein. One unit of enzyme catalyses oxidation of one micromol of NADPH per min under standard mammalian TrxR assay conditions in the presence of 5 mM DTNB.
Notes: Some solution may stick to the rubber cover or stay on the walls of the vial. This enzyme is expressed in E.coli gor mutant and is thus free of glutathione reductase activity. Pure thioredoxin reductase is 35 Units per mg protein and has a molecular mass of 112 000 with two identical subunits. Due to UGA codon acting as stop this preparation contains about 50 percent of the truncated protein lacking the Sec-Gly C-terminus. It is homogenous on Western blots. The truncated protein is folded and does not influence activity.
Stability: 6 months
Storage: Keep enzyme frozen in aliquots at -20 centigrade in the dark.
Reconstitution: Add 1.57 ml of 50 mM Tris-HCl- 1 mM EDTA, pH 7.5 (or other buffer with EDTA) and mix. This will give 2 ml of a solution with 5 Units /ml or 2.8 µM of active enzyme.
Gene Name: Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus ]
Official Symbol: Txnrd1
Synonyms: Tr; thioredoxin reductase 1, cytoplasmic; NADPH-dependent thioredoxin reductase; selenoprotein oxidoreductase; thioredoxin reductase TR1
Gene ID: 58819
mRNA Refseq: NM_031614
Protein Refseq: NP_113802
UniProt ID: O89049
Chromosome Location: 7q13
Pathway: Folic Acid Network, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem
Function: NAD(P)H oxidase activity; flavin adenine dinucleotide binding; mercury ion binding

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