"Sp2/0-Ag14" Related Products

Sp2/0-Ag14 (mouse hybridoma) whole cell lysate

Cat.No.: Sp2-0-Ag14-1677M
Species: Mouse
Tissue Type: B Lymphocyte
Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Size: 200 ug
Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer: In modified RIPA Lysis Buffer.
Applications: Western Blot;Immunoprecipitation;

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