Casein, FITC-conjugated DYE-136

Casein, FITC-conjugated


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Casein, FITC-conjugated

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Cat.No. : DYE-136
Description : Fluoresceinated casein is widely used for fluorometric measurement of protease activity. In the intact substrate, casein is heavily labeled with a fluorescein derivative, resulting in almost total quenching of the conjugate""s fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly labeled fluorescein casein may be useful for a continuous assay if monitored by fluorescence polarization.
Ex(nm) : 494
Em(nm) : 521
Solvent : Water
Storage : Store at -20°C in the dark. Dessicated.

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