Casein, TAMRA-conjugated
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| Cat.No. : | DYE-137 |
| Description : | In the intact substrate, casein is heavily labeled with TAMRA, resulting in almost total quenching of the conjugate"s fluorescence. Compared to the fluoresceinated casein, TAMRA-labeled casein demonstrates pH-insensitive fluorescence. It is readily used for continuous fluorometric measurement of protease activity. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly TAMRA-labeled casein may be useful for a continuous assay if monitored by fluorescence polarization. |
| Ex(nm) : | 545 |
| Em(nm) : | 576 |
| Solvent : | Water |
| Storage : | Store at -20°C in the dark. Dessicated. |
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