Casein, TAMRA-conjugated DYE-137

Cat.No. :	DYE-137
Description :	In the intact substrate, casein is heavily labeled with TAMRA, resulting in almost total quenching of the conjugate"s fluorescence. Compared to the fluoresceinated casein, TAMRA-labeled casein demonstrates pH-insensitive fluorescence. It is readily used for continuous fluorometric measurement of protease activity. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly TAMRA-labeled casein may be useful for a continuous assay if monitored by fluorescence polarization.
Ex(nm) :	545
Em(nm) :	576
Solvent :	Water
Storage :	Store at -20°C in the dark. Dessicated.

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