||Recombinant Human Phospholipase A2 was expressed in E. coli.
||PLA2 (Type V) is a secretory PLA2 (sPLA2) of approximately 14 kDa and is one of the isoforms in the growing list of the PLA2 enzyme family. This enzyme, rather than the sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells. Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in mouse bone marrow-derived mast cells. sPLA2 (Type V) has been cloned from a variety of species including human, mouse, and rat. The enzyme is expressed in heart, lung, placenta, and spleen, as well as P388D1 macrophages and mast cells.
||A solution in 50 mM Tris-HCl, pH 8.0, containing 100 mM sodium chloride, 50 mM calcium chloride, and 20% glycerol
||≥95% estimated by SDS-PAGE
||One unit is defined as the amount of enzyme required to release 1 μmol of TNB per minute under the following conditions: 25 mM Tris-HCl, pH 7.5, containing 10mM CaCl2, 100 mM KCl, 0.3 mM Triton X-100, 1 mg/ml BSA, 1 mM 1,2-bis(heptanoylthio) Glycerophosphocholine, and 0.5 mM DTNB at 37 centigrade.
||6 months at -80 centigrade; Avoid freeze/thaw cycles by aliquoting the protein and storing at -80 centigrade.