Recombinant Human NNMT protein, His-tagged


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Recombinant Human NNMT protein, His-tagged

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Cat.No. : NNMT-148H
Product Overview : Recombinant Human NNMT(Met1-Leu264) fused with His tag at C-terminal was expressed in E. coli.
Description : NNMT (Nicotinamide N­Methyltransferase) is a cytosolic enzyme that catalyzes the N­methylation of nicotinamide and other pyridines using S­adenosyl­L­methionine (AdoMet) as the methyl group donor. The enzyme is highly expressed in liver but is also detected in other organs. NNMT plays a significant role in nicotinamide metabolism and in the detoxification of xenobiotics. The association with thyroid cancer and renal carcinoma makes NNMT useful as a tumor biomarker.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity : Measured by its ability to methylate nicotinamide. The specific activity is >65 pmol/min/µg.
Molecular Mass : Predicted Molecular Mass: 30 kDa;SDS-PAGE: 25-29 kDa, reducing conditions.
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Stability : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name : NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
Official Symbol : NNMT
Synonyms : NNMT; nicotinamide N-methyltransferase;
Gene ID : 4837
mRNA Refseq : NM_006169
Protein Refseq : NP_006160
MIM : 600008
UniProt ID : P40261
Chromosome Location : 11q23.1
Pathway : Biological oxidations, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Methylation, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Phase II conjugation, organism-specific biosystem;
Function : methyltransferase activity; nicotinamide N-methyltransferase activity; transferase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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