Active Recombinant Human NNMT protein, His-tagged

• Cat.No.: NNMT-148H
• Product Overview: Recombinant Human NNMT(Met1-Leu264) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Description: NNMT (Nicotinamide N­Methyltransferase) is a cytosolic enzyme that catalyzes the N­methylation of nicotinamide and other pyridines using S­adenosyl­L­methionine (AdoMet) as the methyl group donor. The enzyme is highly expressed in liver but is also detected in other organs. NNMT plays a significant role in nicotinamide metabolism and in the detoxification of xenobiotics. The association with thyroid cancer and renal carcinoma makes NNMT useful as a tumor biomarker.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to methylate nicotinamide. The specific activity is >65 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 30 kDa;SDS-PAGE: 25-29 kDa, reducing conditions.
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Stability: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
• Official Symbol: NNMT
• Synonyms: NNMT; nicotinamide N-methyltransferase
• Gene ID: 4837
• mRNA Refseq: NM_006169
• Protein Refseq: NP_006160
• MIM: 600008
• UniProt ID: P40261
• Chromosome Location: 11q23.1
• Pathway: Biological oxidations, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Methylation, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Phase II conjugation, organism-specific biosystem
• Function: methyltransferase activity; nicotinamide N-methyltransferase activity; transferase activity