Recombinant Human NNMT protein, His-tagged NNMT-148H

Recombinant Human NNMT protein, His-tagged

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Recombinant Human NNMT protein, His-tagged

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Cat.No. : NNMT-148H
Product Overview : Recombinant Human NNMT(Met1-Leu264) fused with His tag at C-terminal was expressed in E. coli.
Description : NNMT (Nicotinamide N­Methyltransferase) is a cytosolic enzyme that catalyzes the N­methylation of nicotinamide and other pyridines using S­adenosyl­L­methionine (AdoMet) as the methyl group donor. The enzyme is highly expressed in liver but is also detected in other organs. NNMT plays a significant role in nicotinamide metabolism and in the detoxification of xenobiotics. The association with thyroid cancer and renal carcinoma makes NNMT useful as a tumor biomarker.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity : Measured by its ability to methylate nicotinamide. The specific activity is >65 pmol/min/µg.
Molecular Mass : Predicted Molecular Mass: 30 kDa;SDS-PAGE: 25-29 kDa, reducing conditions.
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Stability : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name : NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
Official Symbol : NNMT
Synonyms : NNMT; nicotinamide N-methyltransferase;
Gene ID : 4837
mRNA Refseq : NM_006169
Protein Refseq : NP_006160
MIM : 600008
UniProt ID : P40261
Chromosome Location : 11q23.1
Pathway : Biological oxidations, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Methylation, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Phase II conjugation, organism-specific biosystem;
Function : methyltransferase activity; nicotinamide N-methyltransferase activity; transferase activity;

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