Recombinant Human ATP4A protein, His-tagged


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Recombinant Human ATP4A protein, His-tagged

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Cat.No. : ATP4A-19H
Product Overview : Recombinant Human ATP4A(Leu798~Gly896) fused with His tag at N-terminal was expressed in E. coli.
Description : The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.
Source : E. coli
Species : Human
Tag : His
Form : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Molecular Mass : 12.7kDa
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : SDS-PAGE; WB; ELISA; IP.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
Official Symbol : ATP4A
Synonyms : ATP6A
Gene ID : 495
mRNA Refseq : NM_000704.2
Protein Refseq : NP_000695
MIM : 137216
UniProt ID : P20648
Chromosome Location : 19q13.1
Pathway : Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosystem;Ion transport by P-type ATPases, organism-specific biosystem;Oxidative phosphorylation, organism-specific biosystem;
Function : ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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