Recombinant Human ATP4A protein, His-tagged ATP4A-19H

Recombinant Human ATP4A protein, His-tagged

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Recombinant Human ATP4A protein, His-tagged

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Cat.No. : ATP4A-19H
Product Overview : Recombinant Human ATP4A(Leu798~Gly896) fused with His tag at N-terminal was expressed in E. coli.
Description : The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.
Source : E. coli
Species : Human
Tag : His
Form : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Molecular Mass : 12.7kDa
AA Sequence : MGHHHHHHSG SEF-LTPYLIY ITVSVPLPLG CITILFIELC TDIFPSVSLA YEKAESDIMH LRPRNPKRDR LVNEPLAAYS YFQIGAIQSF AGFTDYFTAM AQEGWFPLLC VG
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : SDS-PAGE; WB; ELISA; IP.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
Official Symbol : ATP4A
Synonyms : ATP6A
Gene ID : 495
mRNA Refseq : NM_000704.2
Protein Refseq : NP_000695
MIM : 137216
UniProt ID : P20648
Chromosome Location : 19q13.1
Pathway : Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosystem;Ion transport by P-type ATPases, organism-specific biosystem;Oxidative phosphorylation, organism-specific biosystem;
Function : ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity;

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