"PPM1A" Related Products

Recombinant Human PPM1A, His-tagged

Cat. No.: PPM1A-66H
Product Overview: Recombinant human PP2Cα was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques,46.6 kDa (418 aa).
Description: Protein phosphatase 2C(PP2Cα) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Cα (amino acids 1-382) was cloned into an E. coli expression vector.
Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
Formulation: Liquid. In 10 mM Tris-HCl, pH7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20%glycerol.
Molecular Weight: 46.6 kDa (418 aa), confirmed by MALDI-TOF
Purity: > 95% by SDS - PAGE
Concentration: 1 mg/ml (determined by Bradford assay)
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Specific Activity: >8,000 units/mg of PP2C alpha
Unit Difinition: One unit will hydrolyze 1nanomole of p-nitrophenyl phosphatate(pNPP) per minute at pH 7.4 at 37℃ using 10mM of substrate
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: PPM1A protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform [ Homo sapiens ]
Synonyms: Protein phosphatase 1A; EC 3.1.3.16, Protein phosphatase 2C isoform alpha; PP2C-alpha; IA; PPM1A; PP2CA; MGC9201; PPP2CA; PP2Ac; RP-C; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; PP2A-alpha; replication protein C; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; FLJ42306; PP2C-ALPHA; PP2C-alpha; protein ph; protein phosphatase1A (formerly 2C), magnesium-dependent, alpha isoform
Gene ID: 5494
mRNA Refseq: NM_021003
Protein Refseq: NP_066283
MIM: 606108
UniProt ID: P35813
Chromosome Location: 14q23.1
Function: hydrolase activity; magnesium ion binding; manganese ion binding; protein C-terminus binding; protein serine/threonine phosphatase activity; signal transducer activity

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