"APOH" Related Products

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Recombinant Human APOH, His-tagged

Cat. No.: APOH-80H
Product Overview: Recombinant Human b2 Glycoprotein-I produced inSf9is a glycosylated, polypeptide chain containing 326 amino acids and having a molecular mass of 38,200 Dalton (excluding glycosylation), 45 kDa total mass. 2GP-1 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Description: β2 Glycoprotein I (β2 GP I) is a serum protein with a single polypeptide chain consisting of 326 amino acids and having a molecular weight of 50 kDa. The protein is also known by the alternative name Apolipoprotein H (ApoH) which gives an indication of its phospholipid binding function. Since the detection of β2 Glycoprotein I as a target of antiphospholipid antibodies in 1990 the discussion about the clinical relevance of β2 GP I is still controversial. It is well established that anticardiolipin antibodies are a very heterogeneous group and that β2 GP I is only one of several potential targets for anticardiolipin antibodies. Nevertheless it is generally accepted that the binding of β2 GP I to anionic lipids or surface is essential for the differential detection of anticardiolipin antibodies associated with autoimmune diseases (versus those associated with infectious diseases like syphilis, tuberculosis, AIDS, etc).
Source: Sf9 insect cells.
Physical Appearance: Sterile Filtered clear solution (1mg/ml).
Purity: Greater than 90% as determined by SDS-PAGE.
Formulation: 2GP-1 is supplied in 16mM HEPES buffer pH-7.2, 200mM NaCl, and 20% glycerol.
Immunological Functions: 1. Binds IgA & IgG-type human auto-antibodies, Auto-antibodies to β2 glycoprotein I recognize conformation dependent and linear epitopes. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including a reference calibrator).
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody and Goodpasture patient sera.
Coating Concentration: 0.5-1 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Gene Name: APOH apolipoprotein H (beta-2-glycoprotein I) [ Homo sapiens ]
Synonyms: APOH; apolipoprotein H (beta-2-glycoprotein I); BG; B2G1; beta-2-glycoprotein I; Beta(2)GPI; B2GPI; Apolipoprotein H; Activated protein C-binding protein; APC inhibitor; Anticardiolipin cofactor; Apo-H
Gene ID: 350
mRNA Refseq: NM_000042
Protein Refseq: NP_000033
UniProt ID: P02749
Chromosome Location: 17q23-qter
MIM: 138700
Function: eukaryotic cell surface binding; glycoprotein binding; heparin binding; lipoprotein lipase activator activity; phospholipid binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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