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Recombinant Human APOH, His-tagged

Cat. No. : APOH-80H
Product Overview : Recombinant Human b2 Glycoprotein-I produced inSf9is a glycosylated, polypeptide chain containing 326 amino acids and having a molecular mass of 38,200 Dalton (excluding glycosylation), 45 kDa total mass. 2GP-1 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Description : β2 Glycoprotein I (β2 GP I) is a serum protein with a single polypeptide chain consisting of 326 amino acids and having a molecular weight of 50 kDa. The protein is also known by the alternative name Apolipoprotein H (ApoH) which gives an indication of its phospholipid binding function. Since the detection of β2 Glycoprotein I as a target of antiphospholipid antibodies in 1990 the discussion about the clinical relevance of β2 GP I is still controversial. It is well established that anticardiolipin antibodies are a very heterogeneous group and that β2 GP I is only one of several potential targets for anticardiolipin antibodies. Nevertheless it is generally accepted that the binding of β2 GP I to anionic lipids or surface is essential for the differential detection of anticardiolipin antibodies associated with autoimmune diseases (versus those associated with infectious diseases like syphilis, tuberculosis, AIDS, etc).
Source : Sf9 insect cells.
Physical Appearance : Sterile Filtered clear solution (1mg/ml).
Purity : Greater than 90% as determined by SDS-PAGE.
Formulation : 2GP-1 is supplied in 16mM HEPES buffer pH-7.2, 200mM NaCl, and 20% glycerol.
Immunological Functions : 1. Binds IgA & IgG-type human auto-antibodies, Auto-antibodies to β2 glycoprotein I recognize conformation dependent and linear epitopes. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including a reference calibrator).
Applications : Western-Blot with monoclonal anti-hexa-His-tag antibody and Goodpasture patient sera.
Coating Concentration : 0.5-1 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Gene Name : APOH apolipoprotein H (beta-2-glycoprotein I) [ Homo sapiens ]
Synonyms : APOH; apolipoprotein H (beta-2-glycoprotein I); BG; B2G1; beta-2-glycoprotein I; Beta(2)GPI; B2GPI; Apolipoprotein H; Activated protein C-binding protein; APC inhibitor; Anticardiolipin cofactor; Apo-H
Gene ID : 350
mRNA Refseq : NM_000042
Protein Refseq : NP_000033
UniProt ID : P02749
Chromosome Location : 17q23-qter
MIM : 138700
Function : eukaryotic cell surface binding; glycoprotein binding; heparin binding; lipoprotein lipase activator activity; phospholipid binding

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