||Recombinant Human MB produced inE.Coliis a non-glycosylated polypeptide chain. The rHuMB has a molecular mass of 17.67 kDa. The rHuMB is purified by proprietary chromatographic techniques.
||Myoglobin is a single-chain globular protein of 153 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It has eight alpha helices and a hydrophobic core. It has a molecular weight of 16,700 daltons, and is the primary oxygen-carrying pigment of muscle tissues. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperativity is a property of multimeric/oligomeric proteins only. Instead, the binding of oxygen by myoglobin is unaffected by the oxygen pressure in the surrounding tissue. Myoglobin is often cited as having an "instant binding tenacity" to oxygen given its hyperbolic oxygen dissociation curve. High concentrations of myoglobin in muscle cells allow organisms to hold their breaths longer.
||Sterile Filtered brownish solution.
||The sterile solution (2mg/ml) contains phosphate-buffered saline (pH 7.4) and 0.05% NaN3.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
|Dimers And Aggregates:
||Less than 1% as determined by silver stained SDS-PAGE gel analysis.
||Less than 0.1 ng/µg (IEU/µg) of rHuMB.
||rHuMB although stable at 15℃ for 2 weeks, should be stored at 4℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please do not freeze.