"OSTN" Related Products

Expand

Recombinant Human Osteocrin, His-tagged

Cat. No. : OSTN-168H
Product Overview : Recombinant Human Osteocrin (His-tagged) produced inE.colicontains 106 amino acids and has a molecular mass of 13.6 kDa.
Description : Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
Source : Escherichia Coli.
Sequences Of Amino Acids : MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG.
Specificity : The amino acid sequence of the recombinant human Osteocrin is 100% homologous to the amino acid sequence of the human Osteocrin without signal sequence.
Formulation : Sterile filtered and lyophilized from 0.5 mg/ml in 5mM TRIS, 25mM NaCl, pH 7.5.
Purification Method : One-step procedure using affinity Ni-NTA chromatography.
Purity : >95 % (SDS-PAGE analyzed).
Reconstitution : Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Applications : Western blotting, ELISA.
Stability/Shelf Life : The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name : OSTN osteocrin [ Homo sapiens ]
Synonyms : OSTN; osteocrin; MUSCLIN; Musclin
Gene ID : 344901
mRNA Refseq : NM_198184
Protein Refseq : NP_937827
MIM : 610280
UniProt ID : P61366
Chromosome Location : 3q29
Function : hormone activity

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.