| Cat. No. : |
OSTN-168H |
| Description : |
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator. |
| Source : |
Escherichia Coli. |
| Sequences Of Amino Acids : |
MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG. |
| Specificity : |
The amino acid sequence of the recombinant human Osteocrin is 100% homologous to the amino acid sequence of the human Osteocrin without signal sequence. |
| Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in 5mM TRIS, 25mM NaCl, pH 7.5. |
| Purification Method : |
One-step procedure using affinity Ni-NTA chromatography. |
| Purity : |
>95 % (SDS-PAGE analyzed). |
| Reconstitution : |
Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Applications : |
Western blotting, ELISA. |
| Stability/Shelf Life : |
The lyophilized protein remains stable until the expiry date when stored at -20°C. |
