||Recombinant Human synaptotagmin I expressed inE.coliwas cloned with a N-terminal-GST tag and a linker. It consists of the human Synaptotagmin I (residues 96-421, swissprot accession P21579). The GST-tag is cleaved during purification. The protein is mutated in the position I367T and contains a deletion of three amino acids 117A, 118L, and 119K. MW = 37.8 kDa.
||Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
||> 95% by SDS-PAGE. The protein was observed as a single band migrating at amolecular weight of (< 45 kDa).
||1.0mg/ml in PBS pH7.3.The concentration is calculated from the absorbance at 280nm (e280 = 31150 M-1 cm-1).
||Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 500 μM.
||-20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.