Active Recombinant Human Interleukin 2
Cat.No. : | IL2-116H |
Product Overview : | Recombinant Human interleukin 2 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 2 sequence) was expressed in modifiedhuman 293 cells. |
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Cat. No. : | IL2-116H |
Description : | Interleukin-2 (IL-2) is a pleotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Additionally, IL-2 promotes the proliferation of antigen-activated T lymphocytes, facilitates the cytolytic activity of natural killer (NK) cells and stimulates anti-tumor activity in monocytes by inducing synthesis of GM-CSF, IL-1b and IL-6. IL-2 also promotes the proliferation of large granular lymphocytes and the growth and differentiation of mitogen activated B lymphocytes in vitro. |
Source : | Human 293 cells. |
Amino Acid Sequence : | APTSSSTK KTQLQL EHLLLDLQ MILNGIN NYKNPKLT RMLTFKFY MPKKATEL KHLQCLEE ELKPLEEVL NLAQSK NFHLRPRD LISNINVIVLELKG SETTF MCEY ADE TATIVEFL NRWITFCQSIISTLT. |
Molecular Mass : | IL-2 migrates as a band between 15 and 18 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted molecular mass of 15.4 kDa. |
PI : | IL-2 separates into a number of isoforms with a pI between 6.0 and 7.4 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted pI of 7.05. |
% Carbohydrate : | Purified IL-2 consists of 0-15% carbohydrate by weight. |
Glycosylation : | IL-2 has O-linked oligosaccharides. |
Purity : | >95%, as determined by SDS-PAGE and visualized by silver stain. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
Activity : | The ED50of IL-2 is typically 0.15- 0.25 ng/ml as measured in a cell proliferation assay using the IL-2 dependent murine CTLL-2 T cell line. |
Gene Name : | IL2 interleukin 2 [ Homo sapiens ] |
Synonyms : | IL2; interleukin 2; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; Aldesleukin; interleukin 2; T cell growth factor |
Gene ID : | 3558 |
mRNA Refseq : | NM_000586 |
Protein Refseq : | NP_000577 |
MIM : | 147680 |
UniProt ID : | P60568 |
Chromosome Location : | 22q13.1 |
Pathway : | Cytokine-cytokine receptor interaction; Endocytosis; Jak-STAT signaling pathway |
Function : | interleukin-2 receptor activity; receptor activity |
Products Types
◆ Lysates | ||
IL2-5234HCL | Recombinant Human IL2 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionIL2 promotes the formation of memory T-cells.
IL2 has a pro-tumorigenic role in the tumor microenvironment.
IL2 synergizes with IL-12 in a mixed cytokine environment.
IL2 upregulates the expression of PD-1 checkpoint inhibitors.
IL2 is rapidly internalized by activated T-cells.
IL2 induces M1 macrophage polarization.
IL2 increases blood-brain barrier permeability.
IL2's affinity for its receptor decreases under hypoxic conditions.
IL2's bioactivity is enhanced by glycosylation.
IL2 promotes the differentiation of Th17 cells.
Customer Reviews (3)
Write a reviewPotent in T-cell activation.
Suitable for cytokine assays.
High stability.
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