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Active Recombinant Human Interleukin 2

Cat.No. : IL2-116H
Product Overview : Recombinant Human interleukin 2 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 2 sequence) was expressed in modifiedhuman 293 cells.
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Cat. No. : IL2-116H
Description : Interleukin-2 (IL-2) is a pleotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Additionally, IL-2 promotes the proliferation of antigen-activated T lymphocytes, facilitates the cytolytic activity of natural killer (NK) cells and stimulates anti-tumor activity in monocytes by inducing synthesis of GM-CSF, IL-1b and IL-6. IL-2 also promotes the proliferation of large granular lymphocytes and the growth and differentiation of mitogen activated B lymphocytes in vitro.
Source : Human 293 cells.
Amino Acid Sequence : APTSSSTK KTQLQL EHLLLDLQ MILNGIN NYKNPKLT RMLTFKFY MPKKATEL KHLQCLEE ELKPLEEVL NLAQSK NFHLRPRD LISNINVIVLELKG SETTF MCEY ADE TATIVEFL NRWITFCQSIISTLT.
Molecular Mass : IL-2 migrates as a band between 15 and 18 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted molecular mass of 15.4 kDa.
PI : IL-2 separates into a number of isoforms with a pI between 6.0 and 7.4 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted pI of 7.05.
% Carbohydrate : Purified IL-2 consists of 0-15% carbohydrate by weight.
Glycosylation : IL-2 has O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Activity : The ED50of IL-2 is typically 0.15- 0.25 ng/ml as measured in a cell proliferation assay using the IL-2 dependent murine CTLL-2 T cell line.
Gene Name : IL2 interleukin 2 [ Homo sapiens ]
Synonyms : IL2; interleukin 2; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; Aldesleukin; interleukin 2; T cell growth factor
Gene ID : 3558
mRNA Refseq : NM_000586
Protein Refseq : NP_000577
MIM : 147680
UniProt ID : P60568
Chromosome Location : 22q13.1
Pathway : Cytokine-cytokine receptor interaction; Endocytosis; Jak-STAT signaling pathway
Function : interleukin-2 receptor activity; receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the impact of IL2 on T-cell memory formation? 04/25/2022

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment? 12/12/2021

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment? 11/22/2021

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors? 06/03/2020

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells? 08/18/2019

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages? 07/24/2019

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability? 07/06/2018

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions? 04/11/2018

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity? 02/19/2017

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells? 04/20/2016

IL2 promotes the differentiation of Th17 cells.

Customer Reviews (3)

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Reviews
04/13/2022

    Potent in T-cell activation.

    07/27/2018

      Suitable for cytokine assays.

      01/07/2018

        High stability.

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