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Recombinant Human Persephin

Cat. No.: PSPN-570H
Product Overview: Recombinant Human Persephin was expressed inE. Coli.
Description: Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and like GNDF, promotes ureteric bud branching. However, in contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons. Recombinant human Persephin is a disulfide-linked homodimer, composed of two 10.3 kDa polypeptide chains (192 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Source: E. Coli.
Amino Acid Sequence: ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRGARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHRWQRLPQLSAA ACGCGG.
Reactivity: Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0ng/ml. Human persephin binds to mammalian GFRa4 with the Kd of 100pM. Other members of the GDBF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRa4.
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human PSP.
Reconstitution: The lyophilized PSP is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 °C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 °C for up to 1 week or at -20 °C for future use.
Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 °C. Reconstituted PSP should be stored in working aliquots at -20 °C. Avoid Frequent Freeze-THAW Cycles.
Supplied: Lyophilized protein from 3mM PBS pH7.2, with no additives.
Gene Name: persephin [Homo sapiens]
Synonyms: persephin; PSP; PSPN
Gene ID: 5623
mRNA Refseq: NM_004158
Protein Refseq: NP_004149
MIM: 602921
UniProt ID: O60542
Chromosome Location: 19p13.3
Pathway: Axon guidance
Function: growth factor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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