Recombinant Human Heparanase

• Cat.No.: HPSE-213H
• Product Overview: Recombinant Human Heparanase-1 is produced as a latent 65 kDa precursor and proteolytically processed to its active form inCHO cell.

Specification

• Cat. No.: HPSE-213H
• Description: Heparanase is an endo β-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor chance of survival.
• Source: CHO.
• Concentration: 1µg /ml.
• Buffer: LDS-PAGE buffer
• Application: Positive control for western blot analysis.
• Storage: Store at –20 ℃, avoid repeated freeze-thaw cycles.
• Publication:
  Functional and structural characterization of a heparanase (2015)
  Metalloglycomics of tris(2,2′-bipyridyl) cobalt and ruthenium compounds (2022)

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens ]
• Synonyms: HPSE; heparanase; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase-1; heparanase exon10-deletion; heparanase exon 9&10 deletion;EC=3.2.-.-; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit
• Gene ID: 10855
• mRNA Refseq: NM_001098540
• Protein Refseq: NP_001092010
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation; Metabolic pathways
• Function: beta-glucuronidase activity; calcium ion binding; hydrolase activity, acting on glycosyl bonds; magnesium ion binding