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Recombinant Human Threonyl-tRNA Synthetase, His-tagged

Cat. No.: TARS-217H
Product Overview: Recombinant Human PL-7 produced inSf9is a glycosylated, polypeptide chain having a molecular mass of 83,312 dalton. Recombinant PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Description: Threonyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.
Source: Sf9 insect cells.
Physical Appearance: Sterile Filtered clear soultion.
Formulation: Recombinant Human PL-7 is supplied at a of 0.5 mg/ml in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.
Purity: Greater than 95% (SDS-PAGE).
Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
Coating Concentration: 0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Gene Name: TARS threonyl-tRNA synthetase [ Homo sapiens ]
Synonyms: TARS; threonyl-tRNA synthetase; ThrRS; MGC9344; Threonyl-tRNA synthetase, cytoplasmic; Threonine--tRNA ligase; EC 6.1.1.3; threonine tRNA ligase 1, cytoplasmic
Gene ID: 1158
mRNA Refseq: NM_152295
Protein Refseq: NP_689508
MIM: 187790
UniProt ID: P26639
Chromosome Location: 5p13.2
Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
Function: ATP binding; ligase activity; nucleotide binding; protein binding; protein homodimerization activity; threonine-tRNA ligase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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