||Recombinant human SLC4A4, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW=51.5kDa.
||Sodium bicarbonate cotransporters (NBCs) mediate the coupled movement of sodium and bicarbonate ions across the plasma membrane of many cells. This is an electrogenic process with an apparent stoichiometry of 3 bicarbonate ions per sodium ion. NBC1 has been identified as a key player for regulation of intracellular pH in several cell types. NBC1 Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
|Amino Acid Sequence:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTE NVEGKPSNLG ERGRARSSTF LRVVQPMFNH SIFTSAVSPA AERIRFILGE EDDSPAPPQL FTELDELLAV DGQEMEWKET ARWIKFEEKV EQGGERWSKP HVATLSLHSL FELRTCMEKG SIMLDREASS LPQLVEMIVD HQIETGLLKP ELKDKVTYTL LRKHRHQTKK SNLRSLADIG KTVSSASRMF TNPDNGSPAM THRNLTSSSL NDISDKPEKD QLKNKFMKKL PRDAEASNVL VGEVDFLDTP FIAFVRLQQA VMLGALTEVP VPTRFLFILL GPKGKAKSYH EIGRAIATLM SDEVFHDIAY KAKDRHDLIA GIDEFLDEVI VLPPGEWDPA IRIEPPKSLP SSDKRKNMYS GGENVQMNGD TPHDGGHGGG GHGDCEELQR TGRFCGGLIK DIKRKAPFFA SDFYDALNIQ.
||Liquid. In 20mM Tris-HCl buffer (pH7.5) containing 0.5mM DTT, 0.1mM PMSF, 10% glycerol.
||>90% by SDS–PAGE.
||0.5mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.