||Recombinant human Phosphoserine Phosphatase was overexpressed inE. coliand purified by conventional chromatography. MW = 25 kDa (225 aa).
||Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the -helical bundle domain, is important to substrate recognition and hydrolysis.
||Liquid. in 20 mM HEPES( pH 7.5) containing 1 mM DTT, 100 mM KCl2.
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method).
|Sequences Of Amino Acids:
||MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AM G GA VPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPGIRELVSRLQERNVQVF LI SG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK V I KLLKEKFH FKKIIMIGDGATDMEACPPA DAFIGFGGNV IRQQVKDNAK WY IT D F VELL GELEE.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.