Recombinant Human Chemokine (C-C motif) Ligand 25

• Cat.No.: CCL25-70H
• Product Overview: Recombinant Human Chemokine (C-C motif) Ligand 25 was expressed inE. Coli.

Specification

• Cat. No.: CCL25-70H
• Description: Human TECK (thymus-expressed chemokine) is a novel CC chemokine that was identified int he thymus of mouse and human. The TECK shows chemotactic activity for activated macrophages, dendritic cells and thymocytes. This rec human TECK is a 14.2 kDa protein containing 127 amino acid residues.
• Source: E. Coli.
• Amino Acid Sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAAIFYLPKRHRK VC GN PK SREV QRAMKLLDAR NKVFAKLHHNMQTFQAGPHA VKKLSSGNSK LS SS K FS NPI SSSRKNVSLLISANSGL.
• Supplied: Lyophilized powder with no additives.
• Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of TECK.
• Reactivity: The reactivity of this rhuman TECK was determined by a chemotaxis assay using human monocytes. Significant chemotaxis was achieved using concentration of 0.1-10ng/ml.
• Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
• Reconstitution: The lyophilized TECK should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 °C for future use.
• Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted TECK should be stored in working aliquots at -20°C. AVOID FREQUENT FREEZE THAW CYCLES.
• Publication:
  Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep (2017)

Gene Information

• Gene Name: chemokine (C-C motif) ligand 25 [Homo sapiens]
• Synonyms: chemokine (C-C motif) ligand 25; TECK; Ckb15; SCYA25; MGC150327; CCL25; small inducible cytokine A25; TECKvar; Ck beta-15; chemokine TECK; thymus expressed chemokine; small inducible cytokine subfamily A (Cys-Cys), member 25; TECKvar; Thymus-expressed chemokine; Small-inducible cytokine A25; Chemokine TECK
• Gene ID: 6370
• mRNA Refseq: NM_005624
• Protein Refseq: NP_005615
• MIM: 602565
• UniProt ID: O15444
• Chromosome Location: 19p13.2
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Signaling by GPCR
• Function: chemokine activity; hormone activity; protein binding