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Recombinant Human Chemokine (C-C motif) Ligand 25

Cat. No.: CCL25-70H
Product Overview: Recombinant Human Chemokine (C-C motif) Ligand 25 was expressed inE. Coli.
Description: Human TECK (thymus-expressed chemokine) is a novel CC chemokine that was identified int he thymus of mouse and human. The TECK shows chemotactic activity for activated macrophages, dendritic cells and thymocytes. This rec human TECK is a 14.2 kDa protein containing 127 amino acid residues.
Source: E. Coli.
Amino Acid Sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAAIFYLPKRHRK VC GN PK SREV QRAMKLLDAR NKVFAKLHHNMQTFQAGPHA VKKLSSGNSK LS SS K FS NPI SSSRKNVSLLISANSGL.
Supplied: Lyophilized powder with no additives.
Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of TECK.
Reactivity: The reactivity of this rhuman TECK was determined by a chemotaxis assay using human monocytes. Significant chemotaxis was achieved using concentration of 0.1-10ng/ml.
Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Reconstitution: The lyophilized TECK should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 °C for future use.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted TECK should be stored in working aliquots at -20°C. AVOID FREQUENT FREEZE THAW CYCLES.
Gene Name: chemokine (C-C motif) ligand 25 [Homo sapiens]
Synonyms: chemokine (C-C motif) ligand 25; TECK; Ckb15; SCYA25; MGC150327; CCL25; small inducible cytokine A25; TECKvar; Ck beta-15; chemokine TECK; thymus expressed chemokine; small inducible cytokine subfamily A (Cys-Cys), member 25; TECKvar; Thymus-expressed chemokine; Small-inducible cytokine A25; Chemokine TECK
Gene ID: 6370
mRNA Refseq: NM_005624
Protein Refseq: NP_005615
MIM: 602565
UniProt ID: O15444
Chromosome Location: 19p13.2
Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Signaling by GPCR
Function: chemokine activity; hormone activity; protein binding

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