Recombinant Human CD14 protein, Fc-tagged CD14-613H

Recombinant Human CD14 protein, Fc-tagged

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Recombinant Human CD14 protein, Fc-tagged

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Cat.No. : CD14-613H
Product Overview : Recombinant Human CD14 (Thr20-Cys352) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Thr20
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 62.2 kDa. The protein migrates as 75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Thr20-Cys352
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD14-613H :

Human CD14, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CD14
Official Symbol : CD14
Synonyms : CD14; CD14 molecule; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
Gene ID : 929
mRNA Refseq : NM_000591
Protein Refseq : NP_000582
MIM : 158120
UniProt ID : P08571

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