Recombinant Human CEACAM1 protein, His-tagged CEACAM1-662H

Recombinant Human CEACAM1 protein, His-tagged

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Recombinant Human CEACAM1 protein, His-tagged

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Cat.No. : CEACAM1-662H
Product Overview : Recombinant Human CEACAM1 (Gln35-Gly428) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1/CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1/CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln35
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 44.2 kDa. The protein migrates as 57-100 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Protein length : Gln35-Gly428
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CEACAM1-662H :

Human CEACAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA of CEACAM1-662H :

Immobilized Human CEACAM-1, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag with a linear range of 0.039-1.25 μg/mL (QC tested).

Gene Name : CEACAM1
Official Symbol : CEACAM1
Synonyms : CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP; carcinoembryonic antigen-related cell adhesion molecule 1; BGP1; CD66a; antigen CD66; CD66a antigen; BGPI
Gene ID : 634
mRNA Refseq : NM_001024912
Protein Refseq : NP_001020083
MIM : 109770
UniProt ID : P13688

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