Recombinant Human CEACAM1 protein, His-tagged
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|Product Overview :||Recombinant Human CEACAM1 (Gln35-Gly428) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1/CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1/CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.|
|Predicted N Terminal :||Gln35|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 44.2 kDa. The protein migrates as 57-100 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.|
|Protein length :||Gln35-Gly428|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>92% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CEACAM1-662H :||
Human CEACAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
|Bioactivity-ELISA of CEACAM1-662H :||
Immobilized Human CEACAM-1, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag with a linear range of 0.039-1.25 μg/mL (QC tested).
|Gene Name :||CEACAM1|
|Official Symbol :||CEACAM1|
|Synonyms :||CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP; carcinoembryonic antigen-related cell adhesion molecule 1; BGP1; CD66a; antigen CD66; CD66a antigen; BGPI|
|Gene ID :||634|
|mRNA Refseq :||NM_001024912|
|Protein Refseq :||NP_001020083|
|UniProt ID :||P13688|
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