Recombinant Human CST5 protein, His-tagged CST5-675H

Recombinant Human CST5 protein, His-tagged

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Recombinant Human CST5 protein, His-tagged

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Cat.No. : CST5-675H
Product Overview : Recombinant Human CST5 (Gly21-Val142) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Cystatin-5 (CST5) is also known as Cystatin-D, is a secreted thermostable protein, which belongs to the cystatin family. CST5 is a cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. CST5 / cystatin-D may play a protective role against proteinases present in the oral cavity.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gly21
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 14.7 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Gly21-Val142
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CST5-675H :

Human Cystatin D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CST5
Official Symbol : CST5
Synonyms : CST5; cystatin D; cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor; MGC71922
Gene ID : 1473
mRNA Refseq : NM_001900
Protein Refseq : NP_001891
MIM : 123858
UniProt ID : P28325

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