Active Recombinant Human EFNA5 protein, His-tagged

• Cat.No.: EFNA5-700H
• Product Overview: Active Recombinant Human EFNA5 protein(Met 1-Asn 203)(NP_001953.1) precursor was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-203 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EPHA5 at 20 μg/ml (100 μl/well) can bind human EFNA4 with a linear ranger of 1.28-32 ng/ml.
• Molecular Mass: The recombinant human Ephrin-A5 consists of 194 amino acids and has a calculated molecular mass of 23 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrin-A5 is approximately 27 kDa due to glycosylation.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Spatial control of membrane receptor function using ligand nanocalipers. (2014)

Gene Information

• Gene Name: EFNA5 ephrin-A5 [ Homo sapiens ]
• Official Symbol: EFNA5
• Synonyms: EFNA5; ephrin-A5; EPLG7; AF1; LERK7; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7; EFL5; RAGS; GLC1M
• Gene ID: 1946
• mRNA Refseq: NM_001962
• Protein Refseq: NP_001953
• MIM: 601535
• UniProt ID: P52803