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Recombinant RSV-F F, His tagged

Cat.No.: F-702V
Product Overview: Recombinant RSV-F F extracellular domain (AAB59858.1) (Met 1-Thr 529), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.
Source: Baculovirus-Insect Cells
Species: RSV-F
Tag: His
Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Molecular Mass: The secreted recombinant human RSV-F comprises 518 amino acids with a predicted molecular mass of 58 kDa. The RSV F0 precursor protein is cleaved into the disulfide-linked F1 and F2 subunits. As a result of glycosylation, the apparent molecular mass of the protein is approximately 45-55 kDa and 18 kDa in SDS-PAGE under reducing conditions, corresponding to the two subunits respectively.
Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.
Gene Name: F Fusion protein (F) [ Respiratory syncytial virus ]
Official Symbol: F
Gene ID: 1494475
Protein Refseq: NP_044596
UniProt ID: O09720

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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