Active Recombinant Human NTRK2, Fc-His-tagged

• Cat.No.: NTRK2-158H
• Product Overview: Active Recombinant Human NTRK2(Met 1-His 430)(NP_001007098.1) was expressed in Human Cells, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc&His
• Protein Length: 1-430 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind mouse BDNF in functional ELISA.
• Molecular Mass: The recombinant human TrkB/Fc is a disulfide-linked homodimer. The reduced monomer consists of 646 amino acids and has a predicted molecular mass of 72 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTrkB/Fc monomer is approximately 110-120 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Peptide selected by phage display increases survival of SH‐SY5Y neurons comparable to brain‐derived neurotrophic factor (2018)

Gene Information

• Gene Name: NTRK2 neurotrophic tyrosine kinase, receptor, type 2 [ Homo sapiens ]
• Official Symbol: NTRK2
• Synonyms: NTRK2; neurotrophic tyrosine kinase, receptor, type 2; BDNF/NT-3 growth factors receptor; TRKB; trkB tyrosine kinase; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B; trk-B; GP145-TrkB
• Gene ID: 4915
• mRNA Refseq: NM_001007097
• Protein Refseq: NP_001007098
• MIM: 600456
• UniProt ID: Q16620

Citation

Peptide selected by phage display increases survival of SH-SY5Y neurons comparable to brain-derived neurotrophic factor.

Journal: Journal of cellular biochemistry    PubMed ID: 30387183    Data: 2022/12/13

Authors: Fatemeh Nafian, Mohammad Javad Rasaee, Babak Kamali Doust Azad

Article Snippet:After four rounds of panning, phage displayed peptides were isolated against recombinant human NTRK2 (Fc‐His NTRK2) (Creative BioMart, New York, NY). . Fc‐His NTRK2 is a fusion protein composed of the extracellular domain of the human NTRK2 receptor (Met1‐His430) (NP_001007098.1, transcript variant b) and the poly histidine‐tagged Fc region of human IgG1 (Figure 1).Fc‐His NTRK2 is a fusion protein composed of the extracellular domain of the human NTRK2 receptor (Met1‐His430) (NP_001007098.1, transcript variant b) and the poly histidine‐tagged Fc region of human IgG1 (Figure 1).