Active Recombinant Human TEK protein, Fc-His-tagged

• Cat.No.: TEK-153H
• Product Overview: Active Recombinant Human TEK protein(NP_000450.2)(Ala23-Lys745) is expressed in HEK293, fused with an Fc, 6His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 23-745 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Human ANGP2 at 1 μg/mL (100 μL/well) can bind Human Tie2 with a linear range of 3.98-467.9 ng/mL. 2. Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD202b (Tie2/Tek) Antibody at 1μg/mL (25 μL/well) can bind Human CD202b (Tie2/Tek) with a linear range of 0.46-33.95 ng/mL.
• Molecular Mass: 107.41 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
• Publications:
  • TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1 (2016)

Gene Information

• Gene Name: TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
• Official Symbol: TEK
• Synonyms: TEK; TEK tyrosine kinase, endothelial; venous malformations, multiple cutaneous and mucosal , VMCM; angiopoietin-1 receptor; CD202b; TIE 2; TIE2; VMCM1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2; VMCM; TIE-2; CD202B
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763

Citation

TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1

Journal: Science Advances    PubMed ID: 27757426    Data: 2016/4/1

Authors: Mohammad B. Hossain, Rehnuma Shifat, Candelaria Gomez-Manzano

Article Snippet:In vitro kinase assays were conducted with 200 ng of purified glutathione S -transferase (GST)–tagged TIE2 active protein (SignalChem, no. T04-11G) or the His-tagged TIE2 with deleted kinase domain (Creative BioMart, no. TEK-153H) in kinase buffer [25 mM MOPS (pH 7.2), 12.5 mM β-glycerol phosphate, 20 mM MgCl 2 , 12.5 mM MnCl2, 2 mM EDTA (pH 8.0), 5 mM EGTA (pH 7.0), and 0.25 mM DTT] with the addition of 20 μM adenosine triphosphate (ATP) for cold reactions, and 10 μM ATP mixed with 10 μCi of [γ- 32 P]ATP for radioactive reactions.. The substrates were added to the reaction mixture and incubated at 37°C for 30 min.The substrates were added to the reaction mixture and incubated at 37°C for 30 min.