||Recombinant human sTNFR1 is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein. It is produced inE.coli.
||TNFR1 belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TNF-alpha or TNF-beta to TNFR1 initiates a signal transduction pathway that results in the activation of the transduction factor NFkB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis.
|Amino Acid Sequence:
||MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.
||Lyophilized powder from a solution of 5 mM Tris pH 8.0. with no additives.
||Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg)>98% by SDS-Page analysis.
||Lyophilized protein should be reconstituted in 5mM Tris pH 8.0 to a concentration of 1mg/ml. to a concentration of 1 mg/ml Allow to set at least 30 minutes at 4℃, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4℃ for 1 week or at -20℃ for long term use. It is recommended that a carrier protein As 0.1% HSA or BSA) or medium serum be added to diluted material.
||Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha, is 0.05 µg/ml.
||The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20℃. Reconstituted sTNFR1 receptor should be stored in working aliquots at - 20℃. Avoid frequent freeze thaw cycles.