||RecombinantHuman MYL12 is produced inE.coli.
||Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells, They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages, Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca+2 Calmodulin regulated light chain kinase (MLCK).
||100µl purified human NMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.
||ELISA, Inhibition Assays, Western Blotting.
||Assayed for phosphorilation by MLCK.
|Storage & Stability:
||Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.