||This recombinant human ApoE2 is a 34.3 kDa protein containing 300 amino acid residues. It is produced inE.coli.
||ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins.
|Amino Acid Sequence:
||MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSE QVQ EELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKCL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH.
||If suitable for use (ie, if this material is not being used labelling), we recommned adding 0.1% protease free BSA to dilution buffers to minimize absorption on containers.
||Lyophilized powder from 20mM Sodium Phosphate, pH7.8 + 0.5mM DTT.
||>90% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of ApoE2.
||Lyophilized ApoE should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4℃, Mix Well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at -20℃ for future use.
||The lyophilized powder is stable at room temperature but it is best stored desiccated below 0℃. Reconstituted ApoE should be stored in working aliquots at -20℃. Avoid frequent freeze thaw cycles.