Recombinant Mouse Cardiotrophin 2

• Cat.No.: Ctf2-952M
• Product Overview: Recombinant mouse Neuropoietin produced inE.Coliis a 19.8 kDa protein containing 183 amino acid residues.

Specification

• Cat. No.: Ctf2-952M
• Description: Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-a component, gp 130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway.
• Source: E.Coli.
• Purity: > 98% by SDS-PAGE.
• Endotoxin Level: < 0.1 ng per μg of Neuropoietin.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biological Activity: The ED₅₀was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.5-0.8 μg/ml.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Neuropoietin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Ctf2 cardiotrophin 2 [ Mus musculus ]
• Synonyms: cardiotrophin 2; NP; Gm494; Ctf2; neuropoietin; LOC244218
• Gene ID: 244218
• mRNA Refseq: NM_198858
• Protein Refseq: NP_942155
• UniProt ID: P83714
• Chromosome Location: 7 F3
• Function: ciliary neurotrophic factor receptor binding; leukemia inhibitory factor receptor binding; cytokine activity