Recombinant Mouse II28A protein
Cat.No. : | II28A-992M |
Product Overview : | Recombinant Mouse II28A protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Source : | E.coli |
Species : | Mouse |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁵ IU/mg. |
Molecular Mass : | Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
Protein length : | 174 |
AA Sequence : | DPVPRATRLPVEAKDCHIAQFKSLS PKELQAFKKAKDAIEKRLLEKDMRC SSHLISRAWDLKQLQVQERPKALQA EVALTLKVWENMTDSALATILGQPL HTLSHIHSQLQTCTQLQATAEPKPP SRRLSRWLHRLQEAQSKETPGCLED SVTSNLFRLLTRDLKCVASGDQCV |
Endotoxin : | Less than 0.1 EU/µg of rMuIFN-λ2/IL-28A as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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