|Cat. No. :
|Product Overview :
||Recombinant Human GDF-15/MIC-1 produced inCell cultureis a disulfide linked homodimeric protein consisting of two 114 amino-acid polypeptide chains.
||GDF-15 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in placenta and to a much lesser extent in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest a role for GDF-15 in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.
||98 % (SDS-PAGE, HPLC).
|Endotoxin level :
||< 0.1 ng per μg of GDF-15/MIC-1.
|Biologically Activity :
||Determined by a cell inhibition assay using DU-145 cells. The expected ED50 for this effect is 1.0-2.0 μg/ml.
||The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted GDF-15/MIC-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃. Avoid repeated freeze-thaw cycles.