Active Recombinant Zea Mays Casein Kinase II Alpha Subunit

• Cat.No.: ACK2-168Z

Specification

• Species: Zea Mays
• Source: E.coli
• Tag: Non
• Description: Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. Similar to protein kinase A, CK2 is a tetramer made up from two different subunits α2β2. The catalytic α-subunit corresponds to the C-subunit of PKA, the non-catalytic β-subunit is unique and differs from the R-subunit of PKA in all known features.The recombinant protein kinase CK2α from Zea mays was expressed in E.coli as a non fusion protein and purified by several chromatography steps (phosphocellulose, Superose 6, Resource Q). It is less prone to degradation than the human counterpart. The α-subunit is constitutively active and suitable for labeling casein kinase 2 αsubstrates. It is shown to be extremely salt sensitive, the highest activity is shown without salt. MW=39.2kDa.
• Form: Supplied in 25mM Tris-HCl pH8.5, 500mM NaCl, 1mM DTT, 500μM PMSF and 50% glycerol.
• Activity: >1U/mg (1 Unit is defined as 1 micromole phosphate transferred to synthetic peptide per min at 30℃).
• Purity: >99% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: LOC542637 casein kinase II alpha subunit [ Zea mays ]
• Synonyms: ACK2; LOC542637; casein kinase II alpha subunit; Casein kinase II subunit alpha
• Gene ID: 542637
• mRNA Refseq: NM_001112162
• Protein Refseq: NP_001105632
• UniProt ID: P28523