Active Recombinant Human High-Mobility Group Box 1
Cat.No. : | HMGB1-1048H |
- Specification
- Gene Information
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Cat. No. : | HMGB1-1048H |
Description : | High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication, and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction, or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release. Recombinant HMG1 is isolated from an E. coli strain that carries the coding sequence of the human HMG1 under the control of a T7 promoter. |
Source : | E.coli. |
Purity : | >95% by SDS-PAGE. |
Form : | Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol. |
Activity : | 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction to super-shift TBP-DNA complex, 20 ng are sufficient for reconstituted transcription assay and 100 ng are sufficient for a protein-protein interaction assay. |
Application : | HMG1 has been applied in in vitro transcription assays, DNA-protein and protein-protein interactions assays. |
Usage : | For in vitro use only. |
Storage : | Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles. |
Gene Name : | HMGB1 high-mobility group box 1 [ Homo sapiens ] |
Synonyms : | HMGB1; high-mobility group box 1; HMG1; HMG3; SBP-1; DKFZp686A04236; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1; High mobility group protein B1; High mobility group protein 1; HMG-1; DKFZp686A04236; OTTHUMP00000190860; OTTHUMP00000018199; high-mobility group (nonhistone chromosomal) protein 1 |
Gene ID : | 3146 |
mRNA Refseq : | NM_002128 |
Protein Refseq : | NP_002119 |
MIM : | 163905 |
UniProt ID : | P09429 |
Chromosome Location : | 13q12 |
Pathway : | Base excision repair |
Function : | DNA bending activity; transcription factor binding |
Products Types
◆ Native Protein | ||
HMGB1-8447B | Active Native Bovine HMGB1 | +Inquiry |
◆ Lysates | ||
HMGB1-2076HCL | Recombinant Human HMGB1 cell lysate | +Inquiry |
HMGB1-1677MCL | Recombinant Mouse HMGB1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThis protein can promote tumor growth, invasion and angiogenesis, and plays an important role in tumor immune evasion.
Yes, HMGB1 protein plays an important role in tumor metastasis, particularly by promoting invasion of tumor cells and migration of the hematopoietic system.
Is involved in inflammatory response, vascular damage, and thrombosis in cardiovascular disease and may be associated with myocardial ischemia-reperfusion injury.
HMGB1 protein can promote the activation, chemotaxis and inflammatory response of immune cells, and is also involved in regulating the balance of autoimmune response.
HMGB1 protein is associated with processes such as chronic inflammation and insulin resistance in diabetes and may be a potential target for treatment and prevention.
The protein is involved in pathological processes such as liver fibrosis, hepatitis, and liver cancer in liver disease and may be a target for therapeutic strategies.
Customer Reviews (3)
Write a reviewBroad storage conditions of HMGB1ins make the experimental process more stable and reliable, which provides a strong guarantee for scientific research.
I found that tHMGB1 exhibited very good stability during storage, which allowed us to perform experiments easily and obtain reliable results.
The anti-contamination ability of HMGB1 was excellent in Western blot, and the contamination problem in the experiment was avoided.
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