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Recombinant Human High-Mobility Group Box 1

Cat. No.: HMGB1-1048H
Description: High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication, and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction, or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release. Recombinant HMG1 is isolated from an E. coli strain that carries the coding sequence of the human HMG1 under the control of a T7 promoter.
Source: E.coli.
Purity: >95% by SDS-PAGE.
Form: Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
Activity: 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction to super-shift TBP-DNA complex, 20 ng are sufficient for reconstituted transcription assay and 100 ng are sufficient for a protein-protein interaction assay.
Application: HMG1 has been applied in in vitro transcription assays, DNA-protein and protein-protein interactions assays.
Usage: For in vitro use only.
Storage: Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles.
Gene Name: HMGB1 high-mobility group box 1 [ Homo sapiens ]
Synonyms: HMGB1; high-mobility group box 1; HMG1; HMG3; SBP-1; DKFZp686A04236; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1; High mobility group protein B1; High mobility group protein 1; HMG-1; DKFZp686A04236; OTTHUMP00000190860; OTTHUMP00000018199; high-mobility group (nonhistone chromosomal) protein 1
Gene ID: 3146
mRNA Refseq: NM_002128
Protein Refseq: NP_002119
MIM: 163905
UniProt ID: P09429
Chromosome Location: 13q12
Pathway: Base excision repair
Function: DNA bending activity; transcription factor binding

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