Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human High-Mobility Group Box 1

Cat.No. : HMGB1-1048H
  • Specification
  • Gene Information
  • Related Products
Cat. No. : HMGB1-1048H
Description : High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication, and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction, or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release. Recombinant HMG1 is isolated from an E. coli strain that carries the coding sequence of the human HMG1 under the control of a T7 promoter.
Source : E.coli.
Purity : >95% by SDS-PAGE.
Form : Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
Activity : 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction to super-shift TBP-DNA complex, 20 ng are sufficient for reconstituted transcription assay and 100 ng are sufficient for a protein-protein interaction assay.
Application : HMG1 has been applied in in vitro transcription assays, DNA-protein and protein-protein interactions assays.
Usage : For in vitro use only.
Storage : Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles.
Gene Name : HMGB1 high-mobility group box 1 [ Homo sapiens ]
Synonyms : HMGB1; high-mobility group box 1; HMG1; HMG3; SBP-1; DKFZp686A04236; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1; High mobility group protein B1; High mobility group protein 1; HMG-1; DKFZp686A04236; OTTHUMP00000190860; OTTHUMP00000018199; high-mobility group (nonhistone chromosomal) protein 1
Gene ID : 3146
mRNA Refseq : NM_002128
Protein Refseq : NP_002119
MIM : 163905
UniProt ID : P09429
Chromosome Location : 13q12
Pathway : Base excision repair
Function : DNA bending activity; transcription factor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
Why is HMGB1 protein associated with tumors? 11/07/2019

This protein can promote tumor growth, invasion and angiogenesis, and plays an important role in tumor immune evasion.

Is HMGB1 protein involved in tumor metastasis? 11/05/2019

Yes, HMGB1 protein plays an important role in tumor metastasis, particularly by promoting invasion of tumor cells and migration of the hematopoietic system.

What is the association between HMGB1 protein and cardiovascular disease? 10/02/2019

Is involved in inflammatory response, vascular damage, and thrombosis in cardiovascular disease and may be associated with myocardial ischemia-reperfusion injury.

What is the effect of HMGB1 protein on the immune system? 08/31/2019

HMGB1 protein can promote the activation, chemotaxis and inflammatory response of immune cells, and is also involved in regulating the balance of autoimmune response.

What role does HMGB1 play in diabetes? 08/21/2019

HMGB1 protein is associated with processes such as chronic inflammation and insulin resistance in diabetes and may be a potential target for treatment and prevention.

Does HMGB1 play a role in liver disease? 08/15/2019

The protein is involved in pathological processes such as liver fibrosis, hepatitis, and liver cancer in liver disease and may be a target for therapeutic strategies.

Customer Reviews (3)

Write a review
Reviews
09/22/2021

    Broad storage conditions of HMGB1ins make the experimental process more stable and reliable, which provides a strong guarantee for scientific research.

    05/02/2020

      I found that tHMGB1 exhibited very good stability during storage, which allowed us to perform experiments easily and obtain reliable results.

      01/11/2019

        The anti-contamination ability of HMGB1 was excellent in Western blot, and the contamination problem in the experiment was avoided.

        Ask a Question for All HMGB1 Products

        Required fields are marked with *

        My Review for All HMGB1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends