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Active Recombinant Human Splicing Factor, Arginine/Serine-rich 1

Cat.No. : SFRS1-1015H
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  • Gene Information
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Cat. No. : SFRS1-1015H
Description : ASF or called SF2, a member of SR protein family, is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating of transcription and pre-mRNA splicing. The human ASF/SF2 wild type protein (residues 1-248) was expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography.
Source : Sf9 insect cells.
Form : Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
Purity : >95% by SDS-PAGE.
Activity : 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction and 100 ng are sufficient for a protein-protein interaction assay.
Application : Recombinant ASF/SF2 protein can be used 1) for in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays.2) for protein-protein interaction assay, and 3) for cell growth and proliferation assays.
Usage : For in vitro use only.
Storage : Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.
Gene Name : SFRS1 splicing factor, arginine/serine-rich 1 [ Homo sapiens ]
Synonyms : SFRS1; splicing factor, arginine/serine-rich 1; ASF; SF2; SF2p33; SRp30a; MGC5228; splicing factor 2; pre-mRNA-splicing factor SF2, P33 subunit; alternate splicing factor; ASF-1; SF2P33; SRp30a
Gene ID : 6426
mRNA Refseq : NM_001078166
Protein Refseq : NP_001071634
MIM : 600812
UniProt ID : Q07955
Chromosome Location : 17q21.3-q22
Function : RNA binding; nucleotide binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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