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||The wild type human brahma-related gene 1 (Brg1) encodes a protein of 1,647 amino acids that contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription regulation. BRG1 is an essential component of the SWI/SNF chromatin remodeling complexes and implicated in multiple functions through its interaction with different proteins, including the tumor suppressor protein pRb, serine-threonine kinase LKB1, and other transcription factors. Although Brg1 involves in chromatin remodeling as complexes with other SWI/SNF proteins, purified BRG1 itself is capable of remodeling mononucleosomes and nucleosomal arrays in vitro. Mutations of Brg1 have been found in multiple tumor cell lines. The wild type human BRG1 was expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity.
||Sf9 insect cells.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
||> 95% by SDS-PAGE.
||50-100 ng are required for in vitro nucleosome remodeling assay and 100 ng are required for a protein-protein interaction assay.
||Recombinant BRG1 can be used 1) for protein-protein interaction assay 2) for in vitro transcription assay 3) for in vitro nucleosome remodeling assay and 4) for cell growth assay.
||For in vitro use only.
||Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.