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Recombinant Human Interleukin 1 Family, Member 9, His-tagged

Cat. No.: IL1F9-160H
Product Overview: Recombinant Human IL1F9 encoding the human IL1F9 (Met1–Asp169) was recombined with His tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=18.7KDa.
Description: IL1F9 functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. It could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-18 receptor agonist and interleukin-1 receptor type I (IL-1R1). That is present in epithelial barriers and takes part in local inflammatory response. IL1F9 highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin it is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. It is up-regulated in lesional psoriasis skin.
Source: E. Coli.
Sequence: Human IL1F9 (Met1–Asp169)1MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND 169.
Purity: >95% in SDS gel.
Formulation: Lyophilized 100 μg human IL1F9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
Application: Cell biology, ELISA.
Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at-70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Gene Name: IL1F9 interleukin 1 family, member 9 [ Homo sapiens ]
Synonyms: IL1F9; interleukin 1 family, member 9; IL1E; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; interleukin-1 homolog 1; interleukin 1-related protein 2;IL-1RP2
Gene ID: 56300
mRNA Refseq: NM_019618
Protein Refseq: NP_062564
MIM: 605542
UniProt ID: Q9NZH8
Chromosome Location: 2q12-q21
Function: cytokine activity; interleukin-1 receptor antagonist activity

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