||Recombinant Human IL1F9 encoding the human IL1F9 (Met1–Asp169) was recombined with His tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=18.7KDa.
||IL1F9 functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. It could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-18 receptor agonist and interleukin-1 receptor type I (IL-1R1). That is present in epithelial barriers and takes part in local inflammatory response. IL1F9 highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin it is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. It is up-regulated in lesional psoriasis skin.
||Human IL1F9 (Met1–Asp169)1MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND 169.
||>95% in SDS gel.
||Lyophilized 100 μg human IL1F9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
||Cell biology, ELISA.
||Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
||Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at-70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.