||Recombinant Human C1QTNF1 encoding the human C1q and tumor necrosis factor related protein 1 (CTRP1) Globular form (Cys141-Pro281P) was recombined with His tag at the N-Terminus. That was expressed inE. Coli, and purified by Ni-NTA column.MW=15.5KDa.
||C1QTNF1 has been shown to interact with Arginine vasopressin receptor 2.
||Human C1q and tumor necrosis factor related protein, Globular Form (Cys141-Pro281P)141CKSHYAAFSV GRKKPMHSNH YYQTVIFDTE FVNLYDHFNM FTGKFYCYVP GLYFFSLNVH TWNQKETYLH IMKNEEEVVI LFAQVGDRSI MQSQSLMLEL REQDQVWVRL YKGERENAIF SEELDTYITF SGYLVKHATE P 281.
||>95% in SDS gel.
||Lyophilized 100 μg human C1QTNF1 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
||Cell biology, ELISA.
||Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
||Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.