||Recombinant Human intelectin 1 (galactofuranose binding) encoding the human encoding the mature human Omentin 1 (Thr19-Arg313) with 6 His tag on the N-Terminus was expressed inE. Coli. This protein was purified by Ni-NTA column. MW=34KD.
||Omentin1is highly expressed in omental adipose tissue. As a secreted novel depot‐specific adipokine, Omentin 1 has no effect on basal glucose uptake but enhances insulin‐stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. decreased omentin 1 levels are associated with increasing obesity and insulin resistance.
||Human Omentin 1 (Thr19‐Arg313).TD EANTYFKEWT CSSSPSLPRS CKEIKDECPS AFDGLYFLRT ENGVIYQTFC DMTSGGGGWT LVASVHENDM RGKCTVGDRW SSQQGSKAVY PEGDGNWANY NTFGSAEAAT SDDYKNPGYY DIQAKDLGIW HVPNKSPMQH WRNSSLLRYR TDTGFLQTLG HNLFGIYQKY PVKYGEGKCW TDNGPVIPVV YDFGDAQKTA SYYSPYGQRE FTAGFVQFRV FNNERAANAL CAGMRVTGCN TEHHCIGGGG YFPEASPQQC GDFSGFDWSG YGTHVGYSSS REITEAAVLL FYR.
||>95% in SDS gel.
||Lyophilized 100 μg human Omentin 1 in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Cell biology, ELISA.
||Store lyophilized protein at -20℃ to -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.