||Recombinant Human neurotrophin 3 encoding the mature human NT-3 (Tyr129- Thr257) with 6 His tag on the N-Terminus was expressed inE. Coli. This protein was purified by Ni-NTA column. MW=16 KD.
||Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene. The protein encoded by this gene, NT-3 is a neurotrophic factor, in the NGF (Nerve Growth Factor)-family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses.
||Mature human NT-3(Tyr129- Thr257)YA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT.
||>95% in SDSgel.
||Lyophilized 100 μg human NT-3 in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
||Add 500μl deionized water or TBS to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Cell biology, ELISA.
||Store lyophilized protein at -20℃ to-70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.