||The Transcription Factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α-subunit), 19 kDa (β-subunit) and 12 kDa (γ-subunit). The α- and β-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ-subunit), retains native TFIIA activity. GST-TFIIA (p19) is isolated from anE. colistrain that carries the coding sequence of TFIIA -subunit under the control of a T7 promoter. GST-TFIIA (p19) has been applied in protein-protein interaction assays.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||100 ng are sufficient for a protein-protein interaction assay.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.