||SC35, a member of SR protein family, is an essential premRNA splicing factor. Phosphorylation on serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly interacts with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, thereby suggesting a potential role of SC35 in coordinating of transcription and pre-mRNA splicing. The human SC35 wild type protein was expressed inBaculovirussystem and purified by an affinity column in combination with FPLC chromatography.
||Sf9 insect cells.
||Liquid. Supplied in 20mM Tris-HCl, pH8.0, 100mM KCl, 0.2mM EDTA, 1mM DTT, 20% glycerol.
||1ng is the amount sufficient for a gel mobility shift assay in a 20μl reaction, 50 and 100ng are sufficient for a protein-protein interaction assay.
||Recombinant SC35 protein can be used: (1) For in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays. (2) For proteinprotein interaction assay. (3) For cell growth and proliferation assays.
||>95% by SDS-PAGE.
||For in vitro use only.
||Quality guaranteed for 12 months store at -80℃. Avoid freeze / thaw cycles.