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Recombinant Human THRA, GST-tagged

Cat. No.: THRA-1199H
Description: Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approx. 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approx. 250 amino acids. The aminoterminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TRα and TRβ isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TRβ gene generates two isoforms, TRβ1 and TRβ2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and Transcription Factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding Protein-associated Factor (PCAF), and CREB-binding Protein (CBP). Recombinant GST-TR is isolated from anE. colistrain that carries the coding sequence of the human TRα1 under the control of a T7 promoter.
Source: E. coli.
Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
Purity: > 95% by SDS-PAGE.
Application: GST-TRα1 has been applied in DNA and protein-protein interaction assays.
Activity: 100 ng are sufficient for a protein-protein interaction assay.
Usage: For in vitro use only.
Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.
Gene Name: THRA thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) [ Homo sapiens ]
Synonyms: THRA; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian);AR7; EAR7; ERBA; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; MGC43240; c-ERBA-1; MGC000261; thyroid hormone receptor, alpha; ERBA-related 7; triiodothyronine receptor; thyroid hormone receptor alpha; avian erythroblastic leukemia viral (v-erb-a) oncogene homolog; EAR-7.1/EAR-7.2; THRA3; C-erbA-alpha; EAR-7; OTTHUMP00000164470; c-erbA-1; Nuclear receptor subfamily 1 group A member 1
Gene ID: 7067
mRNA Refseq: NM_003250
Protein Refseq: NP_003241
MIM: 190120
UniProt ID: P10827
Chromosome Location: 17q21.1
Pathway: Neuroactive ligand-receptor interaction
Function: protein binding; protein heterodimerization activity; protein homodimerization activity; single-stranded RNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription activator activity; transcription factor activity; transcription repressor activity; zinc ion binding; metal ion binding; promoter binding

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