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||Herpesvirus VP16 activates expression of immediate early genes in virally-infected cells. As most other eukaryotic transcriptional activator proteins, VP16 has a modular domain structure: it's N-terminus is involved in DNA-protein interactions, while it's C-terminal 79 amino acids have proven to be an especially potent transactivation domain. VP16 has been shown to bind to TBP, TFIIB, and Replication Factor A. Recombinant GST-VP16 is isolated from anE.colistrain that carries the coding sequence of the fused protein under the control of a T7 promoter. GST-VP16 has been applied in protein-protein interaction assays.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||≥95% by SDS-PAGE.
||100 ng are sufficient for a protein-protein interaction assay.
||For in vitro use only.
||Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.