Cat. No. : |
UL48-1208H. |
Description : |
Herpesvirus VP16 activates expression of immediate early genes in virally-infected cells. As most other eukaryotic transcriptional activator proteins, VP16 has a modular domain structure: it's N-terminus is involved in DNA-protein interactions, while it's C-terminal 79 amino acids have proven to be an especially potent transactivation domain. VP16 has been shown to bind to TBP, TFIIB, and Replication Factor A. Recombinant GST-VP16 is isolated from anE.colistrain that carries the coding sequence of the fused protein under the control of a T7 promoter. GST-VP16 has been applied in protein-protein interaction assays. |
Source : |
E.coli. |
Form : |
Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
Purity : |
≥95% by SDS-PAGE. |
Activity : |
100 ng are sufficient for a protein-protein interaction assay. |
Usage : |
For in vitro use only. |
Storage : |
Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles. |