Recombinant Human Creatine Kinase, Muscle, Type 1 CKM-197H

Recombinant Human Creatine Kinase, Muscle, Type 1


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Recombinant Human Creatine Kinase, Muscle, Type 1

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Cat. No. : CKM-197H
Product Overview : Recombinant Human CKMMITI without C-terminal Lysine on both chains produced inPichia Pastorisis a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMMITI is purified by proprietary chromatographic techniques.
Description : Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP: guanido phosphotransferase protein family.
Source : Pichia Pastoris.
Physical Appearance : Sterile Filtered colourless liquid formulation.
Formulation : The protein (7.14 mg/ml) contains 0.1M Tris-HCl, 0.075M NaCl, 10mM 2-ME, 50% glycerol, 0.1% sodium azide, pH 7.2.
Stability : CKMMITI although stable at 15°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity : The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500IU/mg at 37°C.
Gene Name : CKM creatine kinase, muscle [ Homo sapiens ]
Synonyms : CKM; creatine kinase, muscle; CKMM; M-CK
Gene ID : 1158
mRNA Refseq : NM_001824
Protein Refseq : NP_001815
MIM : 123310
UniProt ID : P06732
Chromosome Location : 19q13.2-q13.3
Pathway : Arginine and proline metabolism; Metabolic pathways; Metabolism of amino acids; creatine kinase-M; creatine kinase M chain; EC
Function : ATP binding; creatine kinase activity; nucleotide binding

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